What is saturation in NMR spectroscopy?

A selective saturation NMR pulse is applied to the receptor to transfer magnetization from receptor through spin diffusion to the nearby (bound) ligand (bottom). Effects are detected as STD signals on the pool of free ligands by rapid exchange of the saturation transfer only if binding occurs.

How does STD NMR work?

The STD NMR method is capable of identifying the binding epitope of a ligand when bound to its receptor protein. Ligand protons that are in close contact with the receptor protein receive a higher degree of saturation, and as a result stronger STD NMR signals can be observed.

What is saturation transfer difference?

Saturation transfer difference (STD) spectroscopy allows to detect transient binding of small molecule ligands to macromolecular receptors. If the smaller molecule ligand binds the receptor, saturation will also spread onto the ligand. The result will be that intensity of the ligand signal will be attenuated.

What is NMR shielding?

NMR spectroscopy is an ideal technique for identifying the structure of molecules using NMR spectroscopy. Higher electron density around hydrogen atoms creates greater opposition to the applied magnetic field. These H atoms are referred to as being shielded.

What is chemical shift BYJU’s?

Chemical shift is characterized as the difference between the resonant frequency of the spinning protons and the signal of the reference molecule.

What is NMR PPT?

Powerpoint Templates Page 3 Introduction:- Nuclear Magnetic Resonance (NMR) is a spectroscopy technique which is based on the absorption of electromagnetic radiation in the radio frequency region 4 to 900 MHz by nuclei of the atoms.

Which H is most Deshielded?

Magnetic Anisotropy: Pi Electron Effects

Proton Type	Effect	Chemical shift (ppm)
C 6H 5-H	highly deshielded	6.5 – 8
C=C-H	deshielded	4.5 – 6
C≡C-H	shielded *	~2.5
O=C-H	very highly deshielded	9 – 10