How many samples do I need for RNA-Seq?

recommended a minimum of five samples per group, based on a variety of RNA-Seq datasets, but both they and others noted that much higher sample numbers are necessary to provide adequate power in samples with high gene dispersion, such as in a population comparison of Caucasian and Nigerian derived cells [10, 15].

How many cells do you need for bulk RNA-Seq?

We only need 100 cells for bulk RNA sequencing.

How many reads for single-cell RNA-seq?

The number of reads usually varies between 30,000 and 150,000 per cell in a typical single-cell RNA sequencing project, so the sequencing depth, and the number of cells per sample, both have a significant impact on the costs of your experiment.

Why is RNA seq so expensive?

Nonetheless, RNA sequencing is still expensive and time-consuming, because it first requires the costly preparation of an entire genomic library — the DNA pool generated from the RNA of cells — while the data itself are also difficult to analyze.

How expensive is genome sequencing?

The estimated cost for advancing the ‘draft’ human genome sequence to the ‘finished’ sequence is ~$150 million worldwide.

Are technical replicates needed for RNA-Seq?

In the days of microarrays, technical replicates were considered a necessity; however, with the current RNA-Seq technologies, technical variation is much lower than biological variation and technical replicates are unneccessary. This article gives some great recommendations for cell line replicates.

How much does single cell sequencing cost?

Single Cell Sequencing

Single Cell Sequencing Service	Pricing Unit	Unit Cost
Gene Expression Capture & Library Prep	Price/capture (up to 10000 cells)	$1,800
VDJ Library Prep	Price/capture	$300
Feature Barcode Library Prep	Price/capture	$300
Sequencing of scRNAseq Gene Expression Libraries	Price/cell (50000 reads/cell)	$0.28

How many reads per cell for 10x?

between 30,000 and 70,000 reads
Typically, we recommend a sequencing depth between 30,000 and 70,000 reads per cell for 10x Genomics projects.

How much does a 10X machine cost?

In April, in anticipation of launching its high-throughput Chromium X, which can process 1 million cells at a time, 10x dropped the price of the base model to $35,000, down from about $50,000.