Can HBSS be autoclaved?

HBSS requires 0.35g/L of sodium bicarbonate and pH adjustment prior to filtration. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Can you autoclave a solution twice?

Though it is not necessary to autoclave media twice because single time autoclaving at 119 degree Celsius is much enough to sterlize it. If you will do it second time then important nutrients will denature, as well as salt concentration will be increased. Never inoculate in the media which is autoclaved twice.

How do you autoclave glucose solution?

autoclave separately. for sugar solution 10 minutes, psi 10, and temperature 121 and for medium 15 minutes 15psi ad 121 temperature. after the autoclaving mix both solution and pour into Petri plate.

Can sodium phosphate buffer be autoclaved?

Sterilization can be performed by filtration or autoclaving. Filter the buffer solution through a 0.22 μm filter into a sterile flask or autoclave for 15 to 20 minutes. Keep the buffer solution at +4°C.

How do you make a HBSS buffer?

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 8 g of NaCl to the solution.
3. Add 400 mg of KCl to the solution.
4. Add 140 mg of CaCl2 to the solution.
5. Add 100 mg of MgSO4-7H2O to the solution.
6. Add 100 mg of MgCl2-6H2O to the solution.
7. Add 60 mg of Na2HPO4-2H2O to the solution.

Can you autoclave sucrose?

This product is soluble in water (500 mg/ml). Solutions can be autoclaved for 15-20 minutes at a maximum of 121 °C.

Can I autoclave buffers?

Most buffers and other salt solutions are autoclaved, because filtration of large volumes is time-consuming and disposable sterile filters are expensive. However, before autoclaving any solution you should always check whether it contains any heat- labile ingredients.

Can you autoclave solidified agar?

Most autoclavable media can be satisfactorily re-melted by autoclaving. If free-steaming is not possible, an autoclave cycle of 5-15 minutes at 121°C may be used. Selective agar products should not be re-melted. Further heating may reduce the selectivity of the medium, as many selective components are heat labile.

Can lactose solution be autoclaved?

Possibly can autoclave it for 15 min at 121C and at 15 Psi. Hi, Use 0.22 micrometer filter for sterilizing your solution.

Can you autoclave lactose?

Lactose is a disaccharide and in every condition glucose, a monosaccharide is a better source of Carbon than lactose. Ideally lactose should not down-regulate the growth when autoclaved.

Does phosphate buffer need to be autoclaved?

PBS can also be prepared by using commercially made PBS buffer tablets or pouches. If used in cell culturing, the solution can be dispensed into aliquots and sterilized by autoclaving or filtration. Sterilization may not be necessary depending on its use. PBS can be stored at room temperature or in the refrigerator.

Does the buffer need to be autoclaved?