What is ARMS PCR or allele specific PCR?

“A method used to detect a single base change or SNP using sequence-specific primers is referred to as ARMS or Allele-specific PCR.” The PCR technique can detect mutations like deletion, duplications, insertion or single base change- SNP.

What are arms primers?

The amplification-refractory mutation system (ARMS) is a simple method for detecting any mutation involving single base changes or small deletions. ARMS is based on the use of sequence-specific PCR primers that allow amplification of test DNA only when the target allele is contained within the sample.

What is allele specific PCR used for?

Allele-specific polymerase chain reaction (AS-PCR) is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism (SNP) effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular …

What is Tetra primer ARMS PCR?

The tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) reaction is a simple and economical method to genotype single-nucleotide polymorphisms (SNPs). It uses four primers in a single PCR and is followed just by gel electrophoresis.

What is endpoint PCR?

End point PCR is the analysis after all cycles of PCR are completed. Unlike qPCR, which allows quantification as template is doubling (exponential phase), end point analysis is based on the platau phase of amplification. The quantification is made by densitometry after exposure to a Xray film.

Why do we use PCR for arms?

The Amplification Refractory Mutation System (ARMS) is an application of PCR in which DNA is amplified by allele specific primers. Since the ARMS PCR is mostly done to identify a mutation or a polymorphism it is also important that it should be able to identify whether the change in DNA is heterozygous or homozygous.

How do you make PCR primers for arms?

The ARMS PCR requires a pair of primers including a common and an ARMS primer….But the ARMS primer has the following special features:

1. The primer is usually 30 bases in length.
2. The nucleotide at the 3′ end of the primer should be complementary to the target nucleotide i.e. G for C or C for G and T for A or A for T.

What is difference between PCR and real time PCR?

What is PCR and how is it different from real time RT–PCR? RT–PCR is a variation of PCR, or polymerase chain reaction. The two techniques use the same process except that RT–PCR has an added step of reverse transcription of RNA to DNA, or RT, to allow for amplification.

Are Naat and PCR the same?

Yes, PCR is the most commonly used NAAT technique for detecting specific nucleic acid in a sample. NAAT techniques such as PCR and TMA are both sensitive and specific for detecting the presence of SARS-CoV-2 in a sample.