Which of the following is true about DNA polymerase?

4. Which of the following is true about DNA polymerase? Explanation: DNA pol can synthesize only a new DNA strand not m-RNA. It can do this in the 5′ to 3′ direction.

How does the DNA polymerase and DNA ligase function in the DNA replication?

On the leading strand, DNA is synthesized continuously, whereas on the lagging strand, DNA is synthesized in short stretches called Okazaki fragments. DNA polymerase I replaces the RNA primer with DNA. DNA ligase seals the gaps between the Okazaki fragments, joining the fragments into a single DNA molecule.

What is the function of the enzyme DNA polymerase?

One of the key molecules in DNA replication is the enzyme DNA polymerase. DNA polymerases are responsible for synthesizing DNA: they add nucleotides one by one to the growing DNA chain, incorporating only those that are complementary to the template.

What is the role of the RNA primers during DNA replication in E coli?

Short fragments of RNA serve as primers that can be extended by DNA polymerase. To form a continuous lagging strand of DNA, the RNA primers must eventually be removed from the Okazaki fragments and replaced with DNA. In E.

What is true about DNA polymerase used in PCR?

Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. This heat-stability makes Taq polymerase ideal for PCR. As we’ll see, high temperature is used repeatedly in PCR to denature the template DNA, or separate its strands.

Which of the following is true about DNA polymerase a it can synthesize DNA in the 5 to 3 direction B it can synthesize DNA in the 3 to 5 direction?

The answer to the question would be, option b. It can synthesize DNA in 5′ to 3′ direction.

How does DNA polymerase add nucleotides?

DNA polymerase will add the free DNA nucleotides using complementary base pairing (A-T and C-G) to the 3′ end of the primer this will allow the new DNA strand to form. Adenine pairs with thymine, thymine with adenine, cytosine with guanine and guanine with cytosine. A primer is needed to start replication.

Which end is DNA polymerase complementary to?

3′ end
DNA polymerase will add the free DNA nucleotides using complementary base pairing (A-T and C-G) to the 3′ end of the primer this will allow the new DNA strand to form. Adenine pairs with thymine, thymine with adenine, cytosine with guanine and guanine with cytosine. A primer is needed to start replication.

What is the product of DNA polymerase?

The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from one original DNA molecule.

Why does DNA polymerase need an RNA primer?

Primers are required for DNA synthesis because no known DNA polymerase is able to initiate polynucleotide synthesis. DNA polymerases are specialized for elongating polynucleotide chains from their available 3′-hydroxyl termini. In contrast, RNA polymerases can elongate and initiate polynucleotides.

Does RNA polymerase require a primer?

RNA polymerase II, the enzyme that synthesizes mRNA from DNA, never requires a primer. The enzyme could synthesize DNA in the absence of a primer, initiating with a dNTP. Furthermore, the enzyme recognizes a specific DNA sequence on the template to initiate de novo DNA synthesis.