What is the relative activity of an enzyme?

Relative activity is the ratio between the activity of your sample of interest and the activity of the control sample and therefore expressed as a percentage. Each of the enzymes needs to be assayed by a method specific to that particular type of enzyme.

What three things can determine the effectiveness of an enzyme?

Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.

What is the rate of enzyme activity?

4 Enzyme reaction rates. Enzyme activity is measured in units which indicate the rate of reaction catalysed by that enzyme expressed as micromoles of substrate transformed (or product formed) per minute.

What is the relationship between the reaction rate and the enzyme concentration?

Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.

How do you calculate relative activity of an enzyme?

Enzyme activity = moles of substrate converted per unit time = rate × reaction volume. Enzyme activity is a measure of the quantity of active enzyme present and is thus dependent on conditions, which should be specified.

What 4 factors affect enzyme activity?

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.

At what pH would the enzyme be most effective?

Enzymes in the stomach, such as pepsin ( which digests protein ), work best in very acid conditions ( pH 1 – 2 ), but most enzymes in the body work best close to pH 7.

How can enzyme activity be determined?

Enzyme activity is usually measured by monitoring the rate of product formation. One of the most widely used techniques is to measure the formation of NAD(P)H from NAD(P)+ spectrophotometrically at 340 nm.

How do enzymes enhance the rate of a reaction?

Enzymes are biological catalysts. Catalysts lower the activation energy for reactions. The lower the activation energy for a reaction, the faster the rate. Thus enzymes speed up reactions by lowering activation energy.