What is the purpose of dilution when performing a WBC count?

Whole blood is diluted with a 3\% acetic acid solution, which hemolyzes mature erythrocytes and facilitates leukocyte counting.

What is the diluting fluid used in WBC counting?

Normal saline should be used to make dilutions for total cell counts. Diluting fluids for WBC counts include: crystal violet/acetic acid. gentian violet/acetic acid.

Why is it important to dilute whole blood so much more for total erythrocyte counts than it is for total leukocyte counts?

1), a dilution of the blood is necessary because the concentration of RBCs is extremely high, i.e., about five million cells per μL in healthy human blood samples. The dilution factor will need to be optimized to maintain accurate counts without increasing the test time significantly.

What is the dilution factor in the WBC pipette?

The chamber must be cleaned and dried and the white cell pipette shaken again to thoroughly mix the diluting fluid and blood. The diluting fluid is 2\% v/v Acetic acid solution. It lyses the red cells, leaving only the WBC visible. The chamber is filled in exactly the same manner as for the red count.

Why the blood dilution of 1 in 20 is obtained for WBC counting instead of 1 in 200 as in RBC and platelet counts?

0.5 part of blood is in 100 parts of fluid or, 1 part of blood is mixed in 200 parts of fluid Thus, dilution factor for RBC counting is 200. 0.5 part of blood is mixed in 10 parts of fluid So, 1 part of blood is in 20 parts of fluid Thus, dilution factor for WBC counting is 20.

Why diluting fluid is used for RBC count?

RBC diluting fluid is isotonic with blood, hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation.

When using a hemocytometer for blood cell counts Why is it important to dilute whole blood so much more for total erythrocyte counts than it is for total leukocyte counts?

Because the cell density is very high, you have to dilute so much that you could do over 200 cell counts!

How many chambers are counted during total WBC?

These are counted in the four large corner squares labeled as WBC and if the number is Y. One large area is 1 x 1 mm, and the depth is 0.1 mm. Total area counted in 4 large squares = 4 x 1 x o.