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What does RNA polymerase 1/2 and 3 do?

What does RNA polymerase 1/2 and 3 do?

RNA polymerase I (RNAPI) transcribes rRNA genes, RNA polymerase II (RNAPII) transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA polymerase III (RNAPIII) transcribes tRNA and 5S rRNA genes. This is in contrast with prokaryotes where a single RNA polymerase is responsible for the transcription of all genes.

What is the difference between Pol 1 and 3?

The main difference between DNA polymerase 1 and 3 is that DNA polymerase 1 is involved in the removal of primers from the fragments and replacing the gap by relevant nucleotides whereas DNA polymerase 3 is mainly involved in the synthesis of the leading and lagging strands.

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What is the difference between polymerase 1 and 2?

DNA polymerase 1, 2 and 3 are prokaryotic DNA polymerases involved in DNA replication. Pol 1 catalyzes the repairing of DNA damages. Pol 2 catalyzes the fidelity and processivity of DNA replication. Pol 3 catalyzes the 5′ to 3′ DNA polymerization.

What is Pol III?

In eukaryote cells, RNA polymerase III (also called Pol III) transcribes DNA to synthesize ribosomal 5S rRNA, tRNA and other small RNAs. The genes transcribed by RNA Pol III fall in the category of “housekeeping” genes whose expression is required in all cell types and most environmental conditions.

How do eukaryotic RNA polymerases I II and III differ from each other how are they similar?

RNA polymerases I and III contain the same two non-identical α-like subunits, whereas polymerase II has two copies of a different α-like subunit. All three polymerases share four other common subunits. In addition, each RNA polymerase contains three to seven unique smaller subunits.

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What does RNA polymerase III do?

RNA polymerase III (Pol III) transcribes various small stable RNAs that are essential in multiple cellular pathways, including pre-mRNA splicing (U6 snRNA) and protein synthesis (5S rRNA, tRNAs)2.

What does DNA pol II do?

Upon binding of substrate, DNA Pol II binds nucleoside triphosphates to maintain the hydrogen bonded structure of DNA. The correct dNTP is then bound and the enzyme complex undergoes conformational changes of subdomains and amino acid residues. These conformational changes allow the rate of repair synthesis to be fast.

What does Pol 1 do in DNA replication?

The physiological function of Pol I is mainly to support repair of damaged DNA, but it also contributes to connecting Okazaki fragments by deleting RNA primers and replacing the ribonucleotides with DNA.

What does RNA polymerase II do?

Eukaryotic RNA polymerase II (pol II) is a 12-subunit DNA-dependent RNA polymerase that is responsible for transcribing nuclear genes encoding messenger RNAs and several small nuclear RNAs (1).

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What does RNA pol III do?

What would happen if DNA polymerase 3 stopped working?

DNA polymerase III would not be able to make a complementary strand. Replication woulds stop. What is the function of DNA helicase? To unwind the double helix by disrupting hydrogen bonds.

How many different RNA polymerases are there in eukaryotes and what types of genes do they transcribe?

All eukaryotes have three different RNA polymerases (RNAPs) which transcribe different types of genes. RNA polymerase I transcribes rRNA genes, RNA polymerase II transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA polymerase III transcribes tRNA and 5S rRNA genes.