What is the difference between DNA polymerase I and DNA polymerase III in terms of their jobs during DNA replication?

DNA polymerase I synthesizes DNA on leading strands and DNA polymerase III synthesizes DNA on lagging strands. DNA polymerase I synthesizes DNA on lagging strands and DNA polymerase III synthesizes DNA on leading strands.

What is a difference between DNA polymerase I and II?

DNA polymerase 1, 2 and 3 are prokaryotic DNA polymerases involved in DNA replication. Pol 1 catalyzes the repairing of DNA damages. Pol 2 catalyzes the fidelity and processivity of DNA replication. Pol 3 catalyzes the 5′ to 3′ DNA polymerization.

What is the role of DNA polymerase 1 in DNA replication?

DNA polymerase I functions to fill DNA gaps that arise during DNA replication, repair, and recombination. DNA polymerase II also functions in editing and proofreading mainly in the lagging strand (Kim et al. 1997, Wagner and Nohmi 2000). DNA polymerase III is the main replicative enzyme.

What is the function of DNA polymerase III?

The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.

What is the difference between RNA polymerase 1/2 and 3?

The main difference between RNA Polymerase 1, 2 and 3 is that the RNA polymerase 1 (Pol 1) transcribes rRNA genes and, the RNA polymerase 2 (Pol 2) mainly transcribes mRNA genes while the RNA polymerase 3 (Pol 3) mainly transcribes tRNA genes.

Does DNA polymerase 1 require 3 Oh?

DNA polymerase cannot synthesize new DNA without a pre-existing 3′-OH. Thus, DNA replication requires an RNA primer to initiate strand formation. (A) First, the PriA protein displaces the SSB proteins.

What is the function of polymerase 3?

What are the three different roles of DNA polymerase?

The three different roles played by DNA polymerase are polymerisation, repair and proofreading.

Which strand is DNA polymerase 3 on?

leading strand
In prokaryotic cells, polymerase III is the major replicative polymerase, functioning in the synthesis both of the leading strand of DNA and of Okazaki fragments by the extension of RNA primers.