What are Rep1 and Rep2 genes in the plasmid used for?

Rep1 and Rep2 and the DNA locus STB form the partitioning system ensuring equal segregation of the plasmid. The Flp recombinase and its target sites FRTs form the amplification system which is responsible for the steady state plasmid copy number.

What is Yep vector?

The YEP vector is episomal in nature as it can integrate with one of the yeast chromosomes. Integration occurs by homologous recombination between the LEU2 gene. This results in the insertion of the entire YEP into on of the yeast chromosome. ADVERTISEMENTS: The plasmid may remain integration or it may excise later.

What is an integrative plasmid?

Integrative plasmids are in most cases suicide vectors, that is, vectors that are unable to replicate in the destination host and therefore must either integrate or disappear, and hence, any plasmid that can be efficiently transferred into the recipient may be used.

What is yeast episomal plasmid?

YEps were first constructed by Beggs (1978) by recombining an E. coli cloning vector with the naturally occurring yeast 2 µm plasmid. The word “episomal” indicates that a YEp can replicate as an independent plasmid, but also implies that integration into one of the yeast chromosomes can occur.

Is YEp13 a shuttle vector?

Plasmid: YEp13 YEp13 is mitotically stable in cir+ strains. [2] A YE type S. cerevisiae/Escherichia coli shuttle vector for cloning specific genes by transformation of yeast.

Can yeast take up plasmids?

Yeast Replicating plasmids (YRp): These vectors contain an Autonomously Replicating Sequence (ARS) derived from the yeast chromosome. As the name suggests, these vectors can replicate independently of the yeast chromosome; however, they tend to be unstable and may be lost during budding.

Do yeast contain plasmids?

The study of yeast DNA plasmids has been initiated with the discovery of the 2-micron DNA in Saccharomyces cerevisiae. This multiple copy plasmid, organized into chromatin structure in vivo, probably exists in the nucleus and provides a good system to obtain information on eukaryotic DNA replication.

What is a centromeric plasmid?

Yeast Centromere plasmids (YCp): These are considered low copy vectors and incorporate part of an ARS along with part of a centromere sequence (CEN). These vectors replicate as though they are small independent chromosomes and are thus typically found as a single copy.

What are the different types of plasmid?

5 types of plasmids are given below.

• Resistance Plasmids.
• Virulence Plasmids.
• Degradative Plasmids.
• Col Plasmids.
• Fertility F Plasmids.

What are centromeric plasmids?

Why is YEp called a shuttle vector?

A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species. Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types.

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