When an alien DNA is linked with the origin of replication so that this alien piece of DNA can replicate and multiply itself in the host organism then this can be called as?

cloning
Thus, an alien DNA is linked with the origin of replication, so that, this alien piece of DNA can replicate and multiply itself in the host organism. This can also be called as cloning or making multiple identical copies of any template DNA.

What is the problem with the presence of more than one cloning sites with in the vector?

Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. Therefore, in order to link the alien DNA (or foreign DNA), the vector needs to have very few, preferably single, recognition/cloning sites for the commonly used restriction enzymes.

What is the method used to introduce alien DNA into plant cells?

Biolistics
Biolistics or gene gun method is suitable for transferring alien DNA into a plant cell.

When an alien DNA is linked with the origin of replication?

Question	When an alien DNA is linked with the origin of replication, so that this alien piece of DNA can replicate and multiply itself in the host organism, then this can be called as
Chapter Name	Biotechnology : Principles And Processes
Subject	Biology (more Questions)
Class	12th
Type of Answer	Video, Text & Image

When an alien DNA is ligated in tetracycline resistance gene The recombinant?

When an alien gene is ligated at the Sal I site of tetracycline resistance gene in the vector pBR322, the recombinant lose tetracycline resistance due to insertion of the foreign DNA.

What does restriction refer to in the restriction endonuclease?

(a) Cleaving of the phosphodiester bond in DNA by the enzyme. Hint: Restriction means to stop and the same applies to the name of this enzyme because it also restricts something that is very important for some unicellular organisms. …

Why there should be one cloning site for one restriction enzyme in a vector?

“Restriction enzymes should not have more than one site of action in the cloning site of a vector. If the restricition enzymes have more than one recognition site in a vector, then the vector itself will get fragmented on treatment on treatment with the restriciton enzyme .

Which of the following methods are used to introduce alien?

(a) Electroporation (chemical method): The bacterial cell is placed in a solution with cold CaCl2 solution followed by placing them at 42°C and then putting them on ice….Question : Write the methods to introduce alien DNA into Host cells.

Question	Write the methods to introduce alien DNA into Host cells.
Class	12th

What is the technique of inserting DNA into plants?

For GM plants, the bacterium most frequently used is called Agrobacterium tumefaciens. The gene of interest is transferred into the bacterium and the bacterial cells then transfer the new DNA to the genome of the plant cells. The plant cells that have successfully taken up the DNA are then grown to create a new plant.