Why are type II restriction enzymes used in cloning?
Table of Contents
- 1 Why are type II restriction enzymes used in cloning?
- 2 What is ROP in pBR322 Class 12?
- 3 What does ROP represent?
- 4 Why are the restriction sites of Type II enzymes usually palindromic?
- 5 What is the recognition sequence of Hind 2?
- 6 Which of the following restriction site is present in ROP gene of pBR 322?
- 7 How many recognition sites is are present in pBR322 and pvu 1?
Why are type II restriction enzymes used in cloning?
Type II restriction enzymes are the familiar ones used for everyday molecular biology applications such as gene cloning and DNA fragmentation and analysis. These enzymes cleave DNA at fixed positions with respect to their recognition sequence, creating reproducible fragments and distinct gel electrophoresis patterns.
What is ROP in pBR322 Class 12?
The different genes present in the vector have different functions. Complete answer: The ‘rop’ gene which is present in the pBR322 codes for the rop protein. Its main function is in decreasing the copy number and so this gene is involved in the replication of the plasmid.
What does ROP represent?
Retinopathy of prematurity (ROP) is known to be an important cause of visual impairment and blindness in the surviving premature population….ROP.
| Acronym | Definition |
|---|---|
| ROP | Retinopathy Of Prematurity |
| ROP | Réseau de Recherche sur les Opérations de Paix (French: Research Network on Peace Operations) |
How many recognition sites are there in pBR322?
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !- lactamase (AmpR) gene and eight unique sites within the TetR gene.
Which recognition site of pBR322 is not useful in genetic engineering?
E. coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracycline.
Why are the restriction sites of Type II enzymes usually palindromic?
Explanation: Enzymes such as restriction enzymes have to recognize a very specific sequence in order to carry out its task. It binds to the DNA only in one specific configuration. A palindromic sequence also increases the chance that both strands of DNA are cut.
What is the recognition sequence of Hind 2?
Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1). Compatible ends Hind II generates fragments with blunt ends and is compatible to any other blunt end. Isoschizomers Hind II is an isoschizomer to Hinc II.
Which of the following restriction site is present in ROP gene of pBR 322?
Rop gene present in pBR 322 codes for the proteins involved in the replication of the plasmid. – BamH 1 site for the restriction enzyme is present on the gene for tetracycline resistance.
What is Stage 2 zone 2 ROP?
Classification system of ROP
| Zone 1 | Defined by a circle whose radius is twice the distance from the centre of the optic disc to the macula. |
|---|---|
| Zone 2 | Defined by a circle whose radius is the distance from the centre of the optic disc to the nasal margin of the retina (ora serrata). |
How can ROP be prevented?
Preventing ROP before delivery A course of steroids, given to mothers likely to give birth prematurely, improves survival and reduces the complications of prematurity, including ROP. Antenatal steroids should be routine for mothers likely to give birth to a baby of less than 35 weeks’ gestation.
How many recognition sites is are present in pBR322 and pvu 1?
The plasmid has unique restriction sites for more than forty restriction enzymes. Eleven of these forty sites lie within the TetR gene.