Why C8 column is used in HPLC?

Generally, it contains octyl carbon chains, which are shorter. Therefore, its hydrophobicity is comparatively low. Also, this makes low retention time of nonpolar molecules in the column. Hence, C8 columns are better for the separation of small organic compounds.

What is difference between C8 and C18?

C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.

What is ODS C18?

Reversed-Phase C18 Columns (ODS) 78. 06. Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety.

What is difference between ODS and C18 column?

The AQ type C18 column, such the ODS-B, has an end-capping that reduces phase collapse greatly, so it can be run in 100\% water if needed. The ODS-A column has a more typical hydrophobic end-capping. Compounds that require more than 50\% organic to elute will be less affected by the hydrophilic end-capping on the ODS-B.

Is C18 column polar or nonpolar?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.

What is the composition of C18 column?

YMC-Triart C18 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart’s combined physical durability and chromatographic performance.

What is C8 and C18 column?

C8 and C18 both refer to the alkyl chain of a bonded face of a column. They are both used in the high performance liquid chromatography system. On the other hand, Octylsilane(C8) has only 8 carbon atoms on the column parking bonded to silica (Si). C18 will tend to retain more than C8.