Why is RNA splicing important?
Table of Contents
Why is RNA splicing important?
Importance of RNA Splicing RNA splicing facilitates the formation of multiple functional mRNAs from a single transcript, which codes for different proteins. It also helps in the regulation of gene expression and protein content of the cell.
What is the importance of splicing prior to translation?
Alternative splicing allows more than one protein to be produced from a gene and is an important regulatory step in determining which functional proteins are produced from gene expression. Thus, splicing is the first stage of post-transcriptional control.
Why is RNA splicing necessary in eukaryotes?
It is necessary in eukaryotic cells because eukaryotic genes contain non coding regions (known as introns) in between coding regions (known as exons). So to make a functional protein from the mRNA, the introns must be removed and this is done by splicing.
How does RNA splicing work?
RNA splicing removes the introns from pre mRNA to produce the final set of instructions for the protein. As DNA is transcribed into RNA it needs to be edited to remove non-coding regions, or introns, shown in green. The spliceosome then cuts the RNA to release the loop and join the two exons together.
How does RNA splicing occur?
During the process of splicing, introns are removed from the pre-mRNA by the spliceosome and exons are spliced back together. If the introns are not removed, the RNA would be translated into a nonfunctional protein. Splicing occurs in the nucleus before the RNA migrates to the cytoplasm.
Why is splicing needed?
For nuclear-encoded genes, splicing occurs in the nucleus either during or immediately after transcription. For those eukaryotic genes that contain introns, splicing is usually needed to create an mRNA molecule that can be translated into protein.
What is needed for RNA splicing?
They can be located in a wide range of genes, including those that generate proteins, ribosomal RNA (rRNA), and transfer RNA (tRNA). Within introns, a donor site (5′ end of the intron), a branch site (near the 3′ end of the intron) and an acceptor site (3′ end of the intron) are required for splicing.
How does splicing take place?