Why are meristems virus free?

Meristem culture is a widely used method for virus eradication from horticultural plants [9]. Because of the uneven distribution of viruses in plants, it is anticipated that meristem would be virus free [32].

Are meristems disease free?

Plant meristem culture is a unique technique to free away various pathogens including viruses, viroides, mycoplasma, bacteria and fungi (Walkey 1978; Pierik 1989; Bhojwani and Razdan 1996). Meristems are frequently devoid of systemic pathogen due to the absence of differentiated conducting tissues.

How do plants become virus free?

Traditionally, thermotherapy of whole plant has been used to obtain virus free plants from infected stock plants, which is a cumbersome process. Moreover, not all viruses are eliminated by this method. Since 1952 shoot meristem-tip culture has become the most popular method for virus eradication.

Which part of plant is free from virus?

The meristem is the plant part that is best suited for making virus-free plants. This is because even when the plant is infected: with a virus, the meristem (apical and axillary) remains infection free; it can be removed. grown in vitro to obtain virus-free plants.

Is axillary meristem virus free?

In virus-infected plants the meristematic tissues in both apical and axillary buds are free of virus because.

How do plants get disease-free?

Tissue culture is used to produce disease-free plants, and genetically engineered plants are initially multiplied using in vitro methods. There are a number of ways to vegetatively propagate plants through tissue culture, such as organogenesis and meristem culture.

Is used to develop virus free plants?

Meristem culture technique is widely used for obtaining virus-free plants.

Which meristem is used in plant tissue culture to obtain virus free?

8 Distribution of Virus Near Apical Meristems For many years, it was thought that most plant viruses were excluded completely from the primary apical meristem; this formed a basis of obtaining virus-free plants (Chapter 14, Section III, A, 3, b).

What is meristem tissue culture?

Meristem culture is defined as the tissue culture technique, which uses apical meristem with 1-3 leaf primordia to prepare clones of a plant by the vegetative propagation. This technique primarily involves the isolation of meristem by applying a V-Shape cut in the stem.

Why meristem is used in tissue culture?

The meristem is free of virus i.e., in tissue culture if the plant is infected with a virus, by removing meristem one can grow virus free plants. …

Why do meristematic tissues lack vacuoles?

Meristematic cells are the cells that divide frequently. These cells need dense cytoplasm and thin cell walls. For this purpose, they have dense cytoplasm and thin cell walls. Due to this reason, meristematic cells lack vacuole.

How are virus resistant crops made?

Non-conventional methods have also been used successfully to confer virus resistance by transferring primarily virus-derived genes, including viral coat protein, replicase, movement protein, defective interfering RNA, non-coding RNA sequences, and protease, into susceptible plants.