Why can DNA polymerase not be used in PCR?

As the polymerase binds to DNA, it adds nucleotide in the direction of 5′ to 3′. Unfortunately, because it disables at a higher temperature, DNA Polymerase is not suitable for a type of replication called Polymerase Chain Reaction (PCR).

Why does PCR need to use a DNA polymerase from the bacterium Thermus aquaticus group of answer choices?

The main reasons that make Thermus aquaticus (Taq) perfect for DNA sequencing are that it’s active across a wide range of temperatures and as such is able to withstand the protein denaturing necessary during PCR so that PCR cycles can be automated, since the polymerase doesn’t need to be added for each cycle.

How does the sliding clamp improve the Processivity of DNA polymerase III?

A DNA clamp, also known as a sliding clamp or β-clamp, is a protein complex that serves as a processivity-promoting factor in DNA replication. As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand.

Why does PCR use DNA polymerase?

DNA polymerase is an essential component for PCR due to its key role in synthesizing new DNA strands. Consequently, understanding the characteristics of this enzyme and the subsequent development of advanced DNA polymerases is critical for adapting the power of PCR for a wide range of biological applications.

What does the sliding clamp protein do?

The sliding clamp is a ring-shaped protein that encircles duplex DNA, binds to the DNA polymerase and tethers it to the DNA template, preventing its dissociation and providing high processivity.

How would a nonfunctional clamp loader affect DNA replication?

It does not break any bonds. Which result would be a consequence of having a nonfunctioning clamp loader in a DNA replication reaction? The polymerase would bind to the wrong area of the DNA. Primers would not be formed.