Can anaerobic bacteria grow in pour plate?

Viable anaerobic bacteria are quantitated by the fractional gram pour plate technique under an anaerobic atmosphere. Caution must be exercised when applying the method since isolates may be pathogenic.

How can anaerobic bacteria be produced?

(Anaerobic bacteria are found today that generate energy by the oxidation of iron Fe2+ compounds to Fe3+, as well as others that generate energy by oxidizing sulfur compounds.

Which process used in pour plate method?

The pour-plate technique Molten agar cooled to 45°C, is poured into a Petri dish containing a specified amount of the diluted sample. Following the addition of the molten-then cooled agar, the cover is replaced, and the plates gently rotated in a circular motion to achieve uniform distribution of microorganisms.

Which method is used to isolate anaerobic bacteria?

Anaerobic bacteria make up a predominant part of the normal human flora. Adequate specimen collection must avoid contamination with this flora. Suitable methods include thoracentesis, transtracheal aspiration, needle and syringe aspiration of closed abscesses, and endocervical aspiration of intrauterine pus.

Why are the surface colonies on a pour plate larger than those inside the agar?

Why are the surface colonies on a pour plate larger than those within the medium? More room to grow on the surface than in the agar. Toxic by-products dilute out on the surface better. More nutrients available on surface as the bacteria grows.

What is the main advantage of the pour plate method over other methods for isolating bacterial colony?

The pour plate technique can be used to determine the number of microbes/mL in a specimen. It has the advantage of not requiring previously prepared plates, and is often used to assay bacterial contamination of food stuffs.

How do anaerobic bacteria survive in oxygen?

Anaerobes, on the other hand, cannot grow in the presence of oxygen. Oxygen is toxic for them, and they must therefore depend on other substances as electron acceptors. They preferentially utilize oxygen as a terminal electron acceptor, but also can metabolize in the absence of oxygen by reducing other compounds.

What is anaerobic technique?

An anaerobic bacteria culture is a method used to grow anaerobes from a clinical specimen. The methods of obtaining specimens for anaerobic culture and the culturing procedure are performed to ensure that the organisms are protected from oxygen.

What is pour plate method in microbiology?

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate. Each colony represents a “colony-forming unit” (CFU).

How do you handle anaerobic bacteria?

The most effective antimicrobials against anaerobic organisms are metronidazole, the carbapenems (imipenem, meropenem and ertapenem), chloramphenicol, the combinations of a penicillin and a beta-lactamase inhibitor (ampicillin or ticarcillin plus clavulanate, amoxicillin plus sulbactam, and piperacillin plus tazobactam …

How do you identify anaerobic and aerobic bacteria?

Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycollate broth:

1. Obligate aerobes need oxygen because they cannot ferment or respire anaerobically.
2. Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.