What happens when dsRNA enters the cell?
Table of Contents
What happens when dsRNA enters the cell?
The dsRNA enters the cells as the embryo develops. This method knocks out the protein of interest through all stages of development. Drosophila is also used as a model organism to study protein function with RNAi by microinjecting dsRNA into Drosophila eggs or cultured cell lines.
How can dsRNA be formed in a cell?
The production of double-stranded RNA (dsRNA) in eukaryotic cells, generally as the result of viral replication or the transcription of transposable elements and repetitive DNA sequences, is known to elicit two types of cellular defense responses.
Where is dsRNA located?
the cytoplasm
Double-stranded RNA is generated in the cytoplasm during replication of positive-strand RNA and DNA viruses.
Why is double-stranded RNA important?
When recognized by the RNA interference (RNAi) pathway, double-stranded RNA (dsRNA) produced in eukaryotic cells results in posttranscriptional gene silencing. In addition, dsRNA can trigger the interferon response as part of the immune response in vertebrates.
How does dsRNA make mRNA?
Double-stranded RNA virus replication Upon infection, the genomic dsRNA is transcribed in mRNAs that will both serve for translation and/or replication. mRNAs translation produces the proteins necessary to ensure replication and encapsidation. Replication occurs in host cytoplasm and converts ss-mRNA to ds-genomic RNA.
How do dsRNA viruses replicate?
As such, many dsRNA viruses undergo replication within their icosahedral capsids. The replicating RNA polymerases are located within the capsid and produce mRNA strands that are extruded from the particle. In this way, the viral dsRNA does not enter the cytoplasm and evades the hosts’ immune system.
What will happen if the double stranded RNA is produced during transcription?
Second, the two RNA molecules if produced simultaneously would be complementary to each other. Hence would form a double stranded RNA. This would prevent RNA from being translated into protein and the exercise of transcription would become a fertile one.
Which virus has dsRNA?
1.47. 2.2. 1 Group III: dsRNA viruses
| Virus family | Examples (common names) | Group |
|---|---|---|
| Picornaviridae | Enterovirus 71, Rhinovirus, Hepatovirus, Cardiovirus, Aphthovirus, Poliovirus, Parechovirus, Erbovirus, Kobuvirus, Teschovirus, Coxsackie, hepatitis A virus | IV |
| Reoviridae | Reovirus, rotavirus, bluetongue virus | III |
Why dsRNA is more stable than Dsdna?
Double-stranded RNA is more stable than DNA because DNA contains one less hydroxyl group than RNA’s ribose.
Which type of virus integrates its genome into the host cell?
A provirus is a virus genome that is integrated into the DNA of a host cell.
Where do dsRNA viruses replicate?