What is the process of RNA splicing?

RNA splicing is a process in molecular biology where a newly-made precursor messenger RNA (pre-mRNA) transcript is transformed into a mature messenger RNA (mRNA). It works by removing introns (non-coding regions of RNA) and so joining together exons (coding regions).

What happens during RNA splicing quizlet?

A large complex made up of (up to 150) proteins and (5) RNA molecules (snRNA) that splices RNA by interacting with the ends of an RNA intron, releasing the intron and joining the two adjacent exons.

What is splicing and what is the purpose of splicing?

Splicing makes genes more “modular,” allowing new combinations of exons to be created during evolution. Furthermore, new exons can be inserted into old introns, creating new proteins without disrupting the function of the old gene. Our knowledge of RNA splicing is quite new.

What is the function of splicing quizlet?

The nuclear transcript that is processed by modification and splicing to give an mRNA. The process of excising introns from RNA and connecting the exons into a continuous mRNA.

What event takes place at the second transesterification step of splicing?

Following rearrangement of snRNPs, the second transesterification step occurs. In this step, the 3′-OH group of the upstream exon ligates to the 5′ end of the downstream exon (3ss), producing an excised intronic lariat and spliced exons.

What is transesterification in RNA splicing?

The bonding of the guanine and adenine bases takes place via a chemical reaction known as transesterification, in which a hydroxyl (OH) group on a carbon atom of the adenine “attacks” the bond of the guanine nucleotide at the splice site.

Why is RNA needed under splicing?

During splicing, introns are removed and exons are joined together. For the eukaryotic genes that contain introns, splicing is required in order to create an mRNA molecule that is capable of being translated into a protein.

What is a lariat in RNA splicing?

Lariats are discarded byproducts of RNA splicing, the process by which genetic instructions for making proteins are assembled. A new study has found hundreds more lariats than ever before, yielding new information about how splicing occurs and how it can lead to disease.

What does the process of transcription involve?

Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA). This complex initiates transcription, and the RNA polymerase begins mRNA synthesis by matching complementary bases to the original DNA strand.

How does splicing affect transcription?

In splicing, some sections of the RNA transcript (introns) are removed, and the remaining sections (exons) are stuck back together. Some genes can be alternatively spliced, leading to the production of different mature mRNA molecules from the same initial transcript.