What is CREB function?

CREB is a transcription factor that regulates diverse cellular responses, including proliferation, survival, and differentiation. CREB is induced by a variety of growth factors and inflammatory signals and subsequently mediates the transcription of genes containing a cAMP-responsive element.

What is CREB in cell signaling?

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. Additionally, CREB signaling is involved in learning and memory in several organisms (4-6).

What does CREB 2 do?

CAMP response element-binding protein 2(CREB2) is also known as Activating Transcription Factor 2 (ATF2). CREB2 is a CREB repressor, which means it inhibits long-term memory formation. CAMP response element-binding (CREB) proteins are transcription factors which bind to sequences of DNA called cAMP response elements.

What is phospho CREB?

The phospho-CREB (S133) kit enables the cell-based quantitative detection of CREB when phosphorylated at Serine 133 as a readout of ERK pathway activation.

What is the size of CREB?

75 kDa
Size-exclusion chromatography suggests CREB is aggregated Unexpectedly, we found that CREB, a 75 kDa dimer in solution, eluted from the HiLoad Superdex 200 column in two contiguous high molecular weight peaks.

What is cAMP in neuroscience?

The second messenger cyclic AMP (cAMP) is a major intracellular mediator of many hormones and neurotransmitters and regulates a myriad of cell functions, including synaptic plasticity in neurons.

How does CREB regulate transcription?

CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), TGACGTCA3,4,5. Phosphorylation of CREB at Ser133 promotes recruitment of the transcriptional co-activator CBP and its PARALOGUE p300 (Fig. 1a)6,7.

What is cAMP neurotransmitter?

Cyclic AMP (cAMP) is an intracellular second messenger to a wide variety of hormones and neurotransmitters. In T cells, elevated cAMP levels antagonize T cell activation by inhibiting T cell proliferation and by suppressing the production of IL-2 and IFN-γ.

Is CREB a kinase?

The MSK1 is a protein kinase expressed in the central nervous system (Arthur et al., 2004) and a major CREB (Ser133) kinase that is direct downstream target of BDNF receptors and the MAPK cascade (Daumas et al., 2017).

Is CREB a coactivator?

The transcription factor CREB binds to a DNA element known as the cAMP-regulated enhancer (CRE). Consistent with its role as a coactivator, CBP augments the activity of phosphorylated CREB to activate transcription of cAMP-responsive genes.

Where is CREB?

nucleus
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin …

How is CREB activated?

CREB proteins are activated by phosphorylation from various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases on the Serine 133 residue. This chain of leucine residues forms the leucine zipper motif. The protein also has a magnesium ion that facilitates binding to DNA.