What is multiplexing in sequencing?

Sample multiplexing, also known as multiplex sequencing, allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on Illumina instruments. Sample multiplexing is useful when targeting specific genomic regions or working with smaller genomes.

How does amplicon sequencing work?

Amplicon sequencing is a method of targeted next generation sequencing that enables you to analyze genetic variation in specific genomic regions. This method uses PCR to create sequences of DNA called amplicons. Amplicon sequencing is typically used for variant detection.

What is DNA sequencing and how does it work?

Sequencing DNA means determining the order of the four chemical building blocks – called “bases” – that make up the DNA molecule. This pairing is the basis for the mechanism by which DNA molecules are copied when cells divide, and the pairing also underlies the methods by which most DNA sequencing experiments are done.

What is pooling in sequencing?

Pool-seq is an alternative cost- and time-effective option in which DNA from several individuals is pooled for sequencing. In particular, sequencing errors confound with the alleles present at low frequency in the pools possibly giving rise to false positive variants.

Does amplicon include primers?

The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction reagents.

What are amplicon libraries?

Alternatively, if the sequence of specific DNA targets is known, PCR amplification of those targets may be used to produce DNA amplicons within the desired size range. These libraries are referred to as amplicon libraries.

Why DNA sequencing is done?

Sequencing is used in molecular biology to study genomes and the proteins they encode. Information obtained using sequencing allows researchers to identify changes in genes, associations with diseases and phenotypes, and identify potential drug targets.

How are DNA sequences useful in DNA fingerprinting?

DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect.