Which of the following is incorrect about a microarray?

1. Which of the following is incorrect about a microarray? Explanation: The entire cDNA population, labeled with fluorescent dyes or radioisotopes, is allowed to hybridize with the oligo probes on the chip. Explanation: The oligonucleotides are called probes that hybridize to labeled cDNA samples.

What is microarray gene expression data?

A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The DNA molecules attached to each slide act as probes to detect gene expression, which is also known as the transcriptome or the set of messenger RNA (mRNA) transcripts expressed by a group of genes.

Why would it be useful to test for the expression of genes like the ABC B2 gene on a microarray?

Why would it be useful to test for the expression of genes like the ABC-B2 gene on a microarray? If the gene is strongly expressed, it would mean that a particular treatment might not work, or might even be harmful to the person taking that drug.

What is measured in a microarray as a proxy to understand gene expression?

DNA microarrays can simultaneously measure the expression level of thousands of genes within a particular mRNA sample. Two DNA strands hybridize if they are complementary to each other, according to the Watson-Crick rules (adenine binds to thymine, cytosine binds to guanine).

Which database is a microarray gene expression database studying in bioinformatics?

YMD — a microarray database for large-scale gene expression analysis. Retrieve and analyze microarray images and accompanying analysis data from hundreds of different investigators.

Which of the given statement is incorrect about microarray analysis?

Which of the given statements is incorrect about Microarray Analysis? Explanation: Microarray analysis is designed to detect global changes in transcription in a genome but does not provide information about the levels of protein products of the genes, which may also be subject to translational regulation.

What microarray means?

Listen to pronunciation. (MY-kroh-uh-RAY) A laboratory tool used to analyze large numbers of genes or proteins at one time. In a microarray, biologic molecules such as DNA, RNA, or protein are placed in a pattern onto a surface such as a glass slide.

What conclusion can you make from microarray data?

Microarray data allows you to determine the level of gene expression in a patient for many genes at one time. In this case, the data provides some insight into which genes may be affected in the development of cancer. 7. In some cancer cells, mRNA is produced (as it would be in a normal cell).

Which step comes first in an experiment using a gene expression microarray?

The first step in using a microarray is to collect healthy and cancerous tissue samples from the patient. This way, doctors can look at what genes are turned on and off in the healthy cells compared to the cancerous cells. Once the tissues samples are obtained, the messenger RNA (mRNA) is isolated from the samples.

How can we measure gene expression?

Gene expression measurement is usually achieved by quantifying levels of the gene product, which is often a protein. Two common techniques used for protein quantification include Western blotting and enzyme-linked immunosorbent assay or ELISA.

How do you evaluate gene expression?

Most of these techniques, including microarray analysis and reverse transcription polymerase chain reaction (RT-PCR), work by measuring mRNA levels. However, researchers can also analyze gene expression by directly measuring protein levels with a technique known as a Western blot.