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How would you differentiate between RNA and DNA using agarose gel electrophoresis?

How would you differentiate between RNA and DNA using agarose gel electrophoresis?

RNA looks more brighter than DNA on agarose gel as it is single stranded and EtBr has more ability to bind to it. The upper first band may be the genomic DNA contamination as gDNA is heaviest. the other two bands may be two forms of RNA as 28S rRNA or 18S rRNA.

Can gel electrophoresis be used for Mrna?

Yes you could see RNA on a agarose gel. Do not use DNA molecular weight, the size do not coresponds. You need to clean the electrophoresis unit with 0.1M NaOH in order to be RNase free. Poly adenylate RNA form a smire.

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How are DNA or RNA molecules visualized on the gel?

What is the function of a ladder in gel electrophoresis? How are DNA or RNA molecules visualized on the gel? A labeled dye that binds to the DNA is added. Click on the electrophoresis machine to have a closer look at the gel.

How does RNA look on a gel?

Intact total RNA run on a denaturing gel will have sharp, clear 28S and 18S rRNA bands (eukaryotic samples). The 28S rRNA band should be approximately twice as intense as the 18S rRNA band (Figure 1, lane 3). This 2:1 ratio (28S:18S) is a good indication that the RNA is completely intact.

How do you know if its RNA or DNA?

There are two differences that distinguish DNA from RNA: (a) RNA contains the sugar ribose, while DNA contains the slightly different sugar deoxyribose (a type of ribose that lacks one oxygen atom), and (b) RNA has the nucleobase uracil while DNA contains thymine.

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What causes separation of DNA bands during electrophoresis?

To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule is negatively charged, therefore when placed in an electric field, DNA fragments will migrate to the positively charged anode.

How do you visualize RNA?

RNA may be seen via hybridization of a reporter molecule, most commonly through FISH or variations thereof. Alternatively, RNA-binding proteins that bind specific sequences may mark an RNA molecule of interest, or an RNA aptamer that fluoresces upon binding of a fluorophore may be incorporated into the target molecule.